Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
![Trifluoroacetic Acid (ca. 0.5mol/L in Water) [Ion-Pair Reagent for LC-MS], TCI America™](https://assets.fishersci.com/TFS-Assets/CCG/Chemical-Structures/chemical-structure-cas-76-05-1.jpg-250.jpg)
Trifluoroacetic Acid (ca. 0.5mol/L in Water) [Ion-Pair Reagent for LC-MS], TCI America™
CAS: 76-05-1 Molecular Formula: C2HF3O2 Molecular Weight (g/mol): 114.02 MDL Number: MFCD00004169 InChI Key: DTQVDTLACAAQTR-UHFFFAOYSA-N Synonym: trifluoroacetic acid,perfluoroacetic acid,trifluoroethanoic acid,trifluoroacetic acid,acetic acid, trifluoro,trifluoroaceticacid,cf3cooh,trifluoro acetic acid,trifluoro-acetic acid,kyselina trifluoroctova PubChem CID: 6422 ChEBI: CHEBI:45892 IUPAC Name: trifluoroacetic acid SMILES: OC(=O)C(F)(F)F
Acetonitrile with 0.035% Trifluoroacetic acid, LabReady™ Solvent Blend, Solstice
CAS: 75-05-8 Molecular Formula: C2H3N Molecular Weight (g/mol): 41.053 InChI Key: WEVYAHXRMPXWCK-UHFFFAOYSA-N Synonym: methyl cyanide,cyanomethane,ethanenitrile,methanecarbonitrile,ethyl nitrile,methane, cyano,acetonitrile,cyanure de methyl,methylkyanid,mecn PubChem CID: 6342 ChEBI: CHEBI:38472 IUPAC Name: acetonitrile SMILES: CC#N
Acetonitrile with 0.1% Trifluoroacetic acid, LabReady™ Solvent Blend, Solstice
CAS: 75-05-8 Molecular Formula: C2H3N Molecular Weight (g/mol): 41.053 InChI Key: WEVYAHXRMPXWCK-UHFFFAOYSA-N Synonym: methyl cyanide,cyanomethane,ethanenitrile,methanecarbonitrile,ethyl nitrile,methane, cyano,acetonitrile,cyanure de methyl,methylkyanid,mecn PubChem CID: 6342 ChEBI: CHEBI:38472 IUPAC Name: acetonitrile SMILES: CC#N
Trifluoroacetic Acid, For protein sequencing, Solstice
CAS: 76-05-1 Molecular Formula: C2HF3O2 Molecular Weight (g/mol): 114.02 MDL Number: MFCD00004169 InChI Key: DTQVDTLACAAQTR-UHFFFAOYSA-N Synonym: trifluoroacetic acid,perfluoroacetic acid,trifluoroethanoic acid,trifluoroacetic acid,acetic acid, trifluoro,trifluoroaceticacid,cf3cooh,trifluoro acetic acid,trifluoro-acetic acid,kyselina trifluoroctova PubChem CID: 6422 ChEBI: CHEBI:45892 IUPAC Name: trifluoroacetic acid SMILES: OC(=O)C(F)(F)F
eMolecules 76-05-1 | Trifluoroacetic acid, biochemical grade | Oakwood Chemical | MFCD00004169 | 114.023 | C2HF3O2 | 99.000 | OC(=O)C(F)(F)F | 1kg | 662699512
Trifluoroacetic acid, biochemical grade | Oakwood Chemical | 76-05-1 | MFCD00004169 | 114.023 | C2HF3O2 | 99.000 | OC(=O)C(F)(F)F | 1kg | 662699512
Proteochem Inc TFA Ampules (Proteomics Grade), 76-05-1, MFCD00004169, 10 amps
MF: C2 H F3 O2, MW: 114.03, Alternative Names: TFA Ampoules; Trifluoroacetic Acid Ampoules; TFA Amps. Trifluoroacetic acid (TFA) is an effective ion pairing agent for HPLC and proteomics LCMS applications which are regularly used to resolve chromatographically similar analytes.
Waters Corp Proteomics Training Standards, Kit
Waters Corporation Proteomics Training Standards, Kit
TARGETMOL CHEMICALS INC LEVOROTATION NIMORAZOLE 5MG
Also available in 1 mg 10 mg 25 mg 50 mg 100 mg 500 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. Levorotation nimorazole phosphate ester TFA is an anti-anaerobic and anti-parasitic agent.
Selleck Chemical LLC Z-LEHD-FMK TFA
Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a cell-permeable competitive and irreversible inhibitor of enzyme caspase-9 which helps in cell survival
Medchemexpress LLC Homoglutathione TFA | 95.0% | 435.37 | C13H20F3N3O8S | 5mg
Homoglutathione (TFA) is a biological molecule
Medchemexpress LLC PZ703b trifluoroacetate | 98.6% | 1714.46 g/mol | C82H103ClF6N10O13S4 | 5 MG
PZ703b TFA is a Bcl-xL-targeting PROTAC degrader provided for laboratory research use. It induces rapid BCL-XL degradation, triggers caspase-3-mediated apoptosis, and inhibits cancer cell proliferation in vitro, including activity in bladder cancer models and hematologic cell lines. Supplied as a trifluoroacetate salt, it is intended for biochemical and cell-based studies only.
- Bcl-xL PROTAC degrader that promotes targeted protein degradation.
- Induces caspase-3-mediated apoptosis in susceptible cell lines.
- Inhibits cell proliferation and can act synergistically with other small-molecule inhibitors in vitro.
- Demonstrated low-nanomolar cellular potency in selected hematologic cell lines.
- High purity (>98%) suitable for biochemical and cell-based assays.
- Available in small milligram pack sizes for research use only.
Medchemexpress LLC 6bK (TFA) | 1774353-12-6 | MFCD30182289 | 99.4% | 871.94 g/mol | C43H56F3N7O9 | 5 MG
6bK TFA is a potent, selective insulin-degrading enzyme (IDE) inhibitor used in preclinical research to study insulin metabolism and glucose tolerance. It shows an in vitro IC50 of approximately 50 nM and has been reported to increase circulating insulin and improve oral glucose tolerance in high-fat-fed mice.
- Potent IDE inhibition (IC50 ≈ 50 nM).
- Selective activity for insulin-degrading enzyme.
- High purity (≈99.4%).
- Available as solid and DMSO solution in multiple pack sizes.
- Supplied with analytical documents (COA, HPLC, LCMS).
- Recommended storage: solid -20°C; in solvent -80°C (6 months) or -20°C (1 month).
Medchemexpress LLC Bio-AMS TFA | 00-00-0 | 98.0% | 685.65 g/mol | C22H30F3N9O9S2 | 5 MG
Bio-AMS TFA is a bacterial biotin protein ligase inhibitor supplied as the trifluoroacetate (TFA) salt for research use. It shows selective activity against Mycobacterium tuberculosis and disrupts fatty acid and lipid biosynthesis.
- Potent biotin protein ligase inhibition.
- Selective activity against Mycobacterium tuberculosis.
- Provided as the TFA salt to aid handling and solubility.
- High purity: 98.0%.
- Molecular weight 685.65 g/mol; formula C22H30F3N9O9S2.
- Available in small research pack sizes, including 5 mg.
- Suitable for biochemical and microbiology research applications.