Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
Trifluoroacetic Acid, Reagents
CAS: 76-05-1 Molecular Formula: C2HF3O2 Molecular Weight (g/mol): 114.02 MDL Number: MFCD00004169 InChI Key: DTQVDTLACAAQTR-UHFFFAOYSA-N Synonym: Perfluoroacetic Acid, Trifluoroethanoic Acid IUPAC Name: trifluoroacetic acid SMILES: OC(=O)C(F)(F)F
Acetonitrile with 0.1% Trifluoroacetic acid, LabReady™ Solvent Blend, Solstice
CAS: 75-05-8 Molecular Formula: C2H3N Molecular Weight (g/mol): 41.053 InChI Key: WEVYAHXRMPXWCK-UHFFFAOYSA-N Synonym: methyl cyanide,cyanomethane,ethanenitrile,methanecarbonitrile,ethyl nitrile,methane, cyano,acetonitrile,cyanure de methyl,methylkyanid,mecn PubChem CID: 6342 ChEBI: CHEBI:38472 IUPAC Name: acetonitrile SMILES: CC#N
Acetonitrile with 0.035% Trifluoroacetic acid, LabReady™ Solvent Blend, Solstice
CAS: 75-05-8 Molecular Formula: C2H3N Molecular Weight (g/mol): 41.053 InChI Key: WEVYAHXRMPXWCK-UHFFFAOYSA-N Synonym: methyl cyanide,cyanomethane,ethanenitrile,methanecarbonitrile,ethyl nitrile,methane, cyano,acetonitrile,cyanure de methyl,methylkyanid,mecn PubChem CID: 6342 ChEBI: CHEBI:38472 IUPAC Name: acetonitrile SMILES: CC#N
Trifluoroacetic Acid, For protein sequencing, Solstice
CAS: 76-05-1 Molecular Formula: C2HF3O2 Molecular Weight (g/mol): 114.02 MDL Number: MFCD00004169 InChI Key: DTQVDTLACAAQTR-UHFFFAOYSA-N Synonym: trifluoroacetic acid,perfluoroacetic acid,trifluoroethanoic acid,trifluoroacetic acid,acetic acid, trifluoro,trifluoroaceticacid,cf3cooh,trifluoro acetic acid,trifluoro-acetic acid,kyselina trifluoroctova PubChem CID: 6422 ChEBI: CHEBI:45892 IUPAC Name: trifluoroacetic acid SMILES: OC(=O)C(F)(F)F
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic acid | 76-05-1 | MFCD00004169 | 10 x 1 mL
Trifluoroacetic acid | Purity: 99% | Mol Wt: 114.02 | 76-05-1 | MFCD00004169 | 10 x 1 mL
Covachem LCMS Grade TFA (Trifluoroacetic acid) 25ml
CovaChem's LCMS Grade Trifluoroacetic acid (TFA) is well suited as a chromatography additive for difficult to separate analytes in HPLC and LCMS applications. CovaChem's LCMS TFA is an ideal additive for separations of peptides in peptide synthesis applications. Trifluoroacetic acid is widely used to separate both protected amino acid chains and deprotected peptides. Due to the amino and carboxylate groups having vastly different pka values, synthetic peptides can be particularly challenging to separate, The HPLC separation of peptides is much more easily achieved with 0.1% Trifluoroacetic acid being present in the mobile phase.
VWR International Trifluoroacetic acid ≥99.8% | 76-05-1 | MFCD00004169 | 1l
Trifluoroacetic acid ≥99.8% | 76-05-1 | MFCD00004169 | 1l
Proteochem Inc TFA Ampules (Proteomics Grade), 76-05-1, MFCD00004169, 10 amps
MF: C2 H F3 O2, MW: 114.03, Alternative Names: TFA Ampoules; Trifluoroacetic Acid Ampoules; TFA Amps. Trifluoroacetic acid (TFA) is an effective ion pairing agent for HPLC and proteomics LCMS applications which are regularly used to resolve chromatographically similar analytes.
Medchemexpress LLC LL-37 amide TFA | 99.3% | 4492.28 | 10 MG
LL-37 amide TFA is a selective agonist of formyl peptide receptor-like FPRL1, effective in inhibiting periodontal pathogens. It functions by activating FPRL1-mediated immune cell chemotaxis and disrupting bacterial cell membrane integrity. This modified form of LL-37 also regulates inflammatory responses, promotes angiogenesis, and shows improved stability and enhanced resistance to enzymatic degradation compared to natural LL-37.
- Effective against periodontal pathogens
- Activates FPRL1-mediated immune cell chemotaxis
- Disrupts bacterial cell membrane integrity
- Regulates inflammatory responses and promotes angiogenesis
- Improved stability and resistance to enzymatic degradation
- Good antibacterial effects against Gram-positive and Gram-negative bacteria
- Used in research for infection-related diseases and wound healing
Medchemexpress LLC Jbsnf-000028 tfa | C13H14F3N3O2 | 25 MG
JBSNF-000028 TFA is an orally active small molecule inhibitor of the tricyclic nicotinamide N-methyltransferase (NNMT). It reduces endogenous MNA levels in U2OS osteosarcoma cells and shows significant anti-obesity and anti-diabetic activities in the diet-induced obesity (DIO) model. This compound is suitable for research into metabolic diseases such as obesity, type 2 diabetes, and non-alcoholic fatty liver disease.
- Inhibits NNMT activity in U2OS cells with an EC50 of 2.5 μM.
- Shows no cytotoxicity against HepG2 cells at concentrations of 10-100 μM.
- Improves glucose and lipid handling in diet-induced obese mice.
- Enhances glucose tolerance in NNMT knockout mice with diet-induced obesity.
Medchemexpress LLC LY117018 TFA 5mg | 2390041-98-0 | 573.58 | C29H26F3NO6S | 5 MG
LY117018 TFA is the trifluoroacetic acid salt of LY117018, a raloxifene analog and selective estrogen receptor modulator reported to exert antiproliferative effects on breast cancer cell lines. It is provided as a research reagent for in vitro and in vivo studies of estrogen receptor signaling and related pathways.
- Selective estrogen receptor modulator with reported antiproliferative activity.
- Soluble in DMSO up to 100 mg/mL; sonication may be required.
- In vivo formulations reported with solubility ≈5 mg/mL using common co-solvent systems.
- Recommended storage: solid sealed and kept away from moisture; in solution store at -80°C (up to 6 months) or -20°C (up to 1 month).
- Molecular formula C29H26F3NO6S; molecular weight 573.58.
Medchemexpress LLC W1131 TFA 10mM | 2740522-79-4 | C₂₅H₂₀F₃NO₈ | 1 ML
W1131 TFA is a potent STAT3 inhibitor and ferroptosis inducer. It demonstrates anti-cancer properties by inhibiting tumor growth and alleviating chemoresistance in gastric cancer models. W1131 TFA regulates critical cellular processes including the cell cycle, DNA damage response, and oxidative phosphorylation, specifically impacting the IL6-JAK-STAT3 and ferroptosis pathways.
- Potent STAT3 inhibitor
- Induces ferroptosis
- Inhibits cancer progression in subcutaneous xenograft, organoid, and PDX models of gastric cancer
- Alleviates cancer cell chemoresistance to 5-FU
- Regulates cell cycle and DNA damage response
- Modulates oxidative phosphorylation
- Impacts IL6-JAK-STAT3 pathway
- Affects ferroptosis pathway